information

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Phage therapy is influenced by:

Phage therapy is influenced by:

Country :
the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: a lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Chronolability
Mutation rate
Phenotypical delay
Phage cocktail
My point of view

From Wikipedia


If the target host* of a phage therapy treatment is not
an animal the term "
biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

"In silico"

From:"Genomics,Proteomics and Clinical Bacteriology", N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.

Tuesday 1 July 2014

2°- Is Cystic fibrosis disease a potential target for Biocontrol?

old post:

Sunday, 18 July 2010


From:

Microbial ecology of the cystic fibrosis lung*

Haemophilus influenzae
Staphylococcus aureus
Pseudomonas aeruginosa
Burkholderia cepacia complex (BCC)



Venn diagram showing reported coinfections of the CF airways. (NB: coinfection does not necessarily imply direct interaction between species.)
A, Aspergillus spp.; AV, adenovirus;AX, A. xylosoxidans; BP, bacteriophage; C, Candida spp.; Ent, enterobacteria; IPV, influenza and/or parainfluenza virus; K, Klebsiella spp.;
M, mycoplasma; MA, Mycobacterium abscessus; N, Neisseria spp.; OF, oropharyngeal flora; RSV,respiratory syncytial virus; SM, S. maltophilia.


From Wikipedia: Metagenomics
"Metagenomics is the study of metagenomes, genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. Traditional microbiology and microbial genome sequencing rely upon cultivated clonal cultures. This relatively new field of genetic research enables studies of organisms that are not easily cultured in a laboratory as well as studies of organisms in their natural environment.[1]
Early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods.[2] Recent studies use "shotgun" Sanger sequencing or massively parallel pyrosequencing to get (mostly) unbiased samples of all genes from all members of sampled communities.[3]"
From:

Metagenomic Analysis of Respiratory Tract DNA Viral Communities in Cystic Fibrosis and Non-Cystic Fibrosis Individuals*

 

                           


"We obtained sequences from sputum DNA viral communities in
5 individuals with cystic fibrosis (CF) and 5 individuals without the disease. Overall, diversity of viruses in the airways was low, with an average richness of 175 distinct viral genotypes. The majority of viral diversity was uncharacterized.CF phage communities were highly similar to each other, whereas Non-CF individuals had more distinct phage communities, which may reflect organisms in inhaled air.
CF eukaryotic viral communities were dominated by a few viruses, including human herpesviruses and retroviruses.

From:

Deciphering the role of phage in the cystic fibrosis airway*



 

"There was a striking difference in metabolic functions encoded by phage in CF versus Non-CF individuals.Regardless of which viral taxa were present,CF-associated phage shared a common core metabolism that reflected the disease state and aberrant airway physiology".

From:
Mathematical Modeling of Cystic Fibrosis Ecology*


 
"A complete mathematical model of CF would need equations that follow the concentrations of all different kinds of bacterial and phage species in each compartment"


See
QUORUM SENSING*


Comment*
For phages and bacteria the human body is only a component of their world in which at all times and on all occasions they are fighting.