Phage therapy subjects from Sellano town : Are there Mycobacteriophages utilizable for Phage Therapy in Buruli ulcer?

From Wikipedia

If the target host* of a phage therapy treatment is not an animal the term "biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

"In silico"
From:"Genomics,Proteomics and Clinical Bacteriology", N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.

Tuesday, 22 July 2014

Are there Mycobacteriophages utilizable for Phage Therapy in Buruli ulcer?

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Comment: I am happy about this work but not for mycobacteriophage choice. The same phages are used in all works with mycobacteria (the host range for D29 is very large and includes both slow and fast-growing species). This aspect ( this is my opinion) is not a favourable point for phage therapy in Buruli: we must wait for a specific phage for Mycobacterium ulcerans strain that is isolated together in the same country.
This work supports the timeliness of phage therapy before the infection to become chronic (33 days) and confirms my working hypothesis: a, b, c

At the moment my answer to this question is negative.

Mostly well-known mycobacteriopages are isolated and multiplied by growth on Mycobacterium smegmatis lawn and are also lysogenic phages, example:

Mycobacterium phage 244
Mycobacterium phage Adjutor
Mycobacterium phage Angel
Mycobacterium phage BPs
Mycobacterium phage Barnyard
Mycobacterium phage Bethlehem
Mycobacterium phage Boomer
Mycobacterium phage Brujita
Mycobacterium phage Butterscotch
Mycobacterium phage Bxb1
Mycobacterium phage Bxz1
Mycobacterium phage Bxz2
Mycobacterium phage Cali
Mycobacterium phage Catera
Mycobacterium phage Chah
Mycobacterium phage Che12
Mycobacterium phage Che8
Mycobacterium phage Che9c
Mycobacterium phage Che9d
Mycobacterium phage Cjw1
Mycobacterium phage Cooper
Mycobacterium phage Corndog
Mycobacterium phage D29
Mycobacterium phage DD5
Mycobacterium phage Fruitloop
Mycobacterium phage Giles
Mycobacterium phage Gumball
Mycobacterium phage Halo
Mycobacterium phage Jasper
Mycobacterium phage KBG
Mycobacterium phage Konstantine
Mycobacterium phage Kostya
Mycobacterium phage L5
Mycobacterium phage Llij
Mycobacterium phage Lockley
Mycobacterium phage Myrna
Mycobacterium phage Nigel
Mycobacterium phage Omega
Mycobacterium phage Orion
Mycobacterium phage PBI1
Mycobacterium phage PG1
Mycobacterium phage PLot
Mycobacterium phage PMC
Mycobacterium phage Pacc40
Mycobacterium phage Phaedrus
Mycobacterium phage Phlyer
Mycobacterium phage Pipefish
Mycobacterium phage Porky
Mycobacterium phage Predator
Mycobacterium phage Pukovnik
Mycobacterium phage Qyrzula
Mycobacterium phage Ramsey
Mycobacterium phage Rizal
Mycobacterium phage Rosebush
Mycobacterium phage ScottMcG
Mycobacterium phage Solon
Mycobacterium phage Spud
Mycobacterium phage TM4
Mycobacterium phage Troll4
Mycobacterium phage Tweety
Mycobacterium phage U2
Mycobacterium phage Wildcat

To find "genuine" lytic mycobacteriophages is not easy and it seems to me that it is also rare .

D29 is a lytic phage but I can not consider this phage a "genuine " lytic phage by genome analysis.

These mycobacteriophages have a broad Host range and this feature is not favourable when they are used in Phage therapy.

I am of the opinion that Mycobacterium marinum may be the valid alternative to Mycobacterium smegmatis for growing and isolating phages with a potential action on Mycobacterium ulcerans but until now there is little information about growing phages on Mycobacterium marinum.

It is indispensable to work on this virgin ground.

Monitoring

Year 2010 (2011,2012,2013,2014,2015,2016,2017,2018..)

- At present there are Strict rules about Drugs.

Positive and continuous trend in the request of new anti-bacterial drugs.

Year 2025?- Drug regulations changes by a swing in public Opinion and Phage Therapy Units starting inside the Hospitals.

I have read a lot of scientific studies concerning different topics about Phage Therapy and many opinions from the academic world. These comments about the use of Phages in therapy are often sceptical and also sarcastic.
I share some their opinion but the trend of antibiotic resistance is characterising by a relentless advance. In the absence of new antibiotics we are obliged to act with fairness and to weigh the pros and cons of Phage therapy.

Phage Therapy is better than nothing.

Sellano is touched by History

Potential application of Mycobacterium phages in Buruli disease

I have been studying since January 2009 if Phage Therapy could be considered in Buruli ulcer. Patients shed large numbers of bacilli from their wounds.
"In the first stage of disease Mycobacterium ulcerans bacilli are typically found extracellularly, where it might be immediately accessible by phages."
Children under the age of 15 years ( range 2-14 years) are predominantly affected.
After my trip in Ivory Coast it is my personal project and I am exploring if this working hypothesis is possible.

Theory and Practical Applications of Phage Therapy in Human Field.

I must remember that Phage Therapy is not equivalent to the Antibiotic Therapy but in some pathological situations, with well definite circustances and when we have a bacterial infection caused by bacteria resistant to all antibiotics, in that case Phage Therapy would remain the unique and possible treatment with a tangible chance of succes.

A complete and schematized process for working with Phages by Phage Cocktails. Phage Therapy is praticable both in Veterinary Science and in Agricultural and Food Industry. Phage Therapy in Human field is, on the contrary, a demanding job.

I'd like to explain several key points of Phage Therapy, for example:

How to prepare Phages
How to select Phages
How to purify Phages
How to administer Phages

Patenting Issues and Defense of Intellectual Property
One relevant factor in the development of all bacteriophage-based therapies is their intellectual property status, because the idea of using bacteriophages as therapeutic tools is almost a hundred years old, and as such , unpatentable.

Main Obstacles to Phages Therapy

• Pervasive fixation on chemical antibiotics within the clinical establishment.

• Resistance of the pharmaceutical sector, which is heavily invested in chemical antibiotics.

• Physicians' reluctance to forego wide-spectrum antibiotics in favor of the highly specific phages.

• Structural and functional reorganization required for a coordinated and responsive diagnosis-production-administration chain.

• Pervasive aversion within the biotech research establishment to revert to "archaic" microbiology.

• Need to constantly adapt and refresh phage preparations in response to pathogen evolution.

• Delay between clinical presentation and antibacterial administration.

• General disregard for Russian research and clinical practices.

• Concerns with bacterial evolution of resistance to phages.

• Lack of a regulatory reference basis.

Phage therapy subjects from Sellano town

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Tuesday, 22 July 2014

Are there Mycobacteriophages utilizable for Phage Therapy in Buruli ulcer?

The authentic Sellano's gonfalon (the year of 1936)

Sellano's gonfalon

The last story from Selllano's castle and its territory.Click on this image:

Phage Therapy Light and Shade

Potential application of Mycobacterium phages in Buruli disease

Plagiarism?No!But,from the Net to the Net!

Sellano's coat of arms.Click on this image:

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