information

Whoever comes in this website may find a hint

Phage therapy is influenced by:

Phage therapy is influenced by:

Country :
the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: a lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Chronolability
Mutation rate
Phenotypical delay
Phage cocktail
My point of view

From Wikipedia


If the target host* of a phage therapy treatment is not
an animal the term "
biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

"In silico"

From:"Genomics,Proteomics and Clinical Bacteriology", N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.

Saturday, 23 August 2014

The disassembly of the thymidylate synthaseThyX protein model of Mycobacterium ulcerans Agy99

By this site I have worked the sequence of thymidylate synthase ThyX of Mycobacterium ulcerans Agy99 and by Discovery  Studio 4.0 software with the PDB file of the protein sequence. After I have compared  this protein with gp48 D29p06 protein of Mycobacteriophage D29
From SnapGene
" flavin dependent thymidylate synthase; ThyX; thymidylate synthase complementing protein; catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate; the enzyme from Mycobacterium tuberculosis forms homotetramers; uses FAD as a cofactor"


thymidylate synthase ThyX


thymidylate synthase ThyX with chain A highlighted




thymidylate synthase ThyX without chain A highlighted


thymidylate synthase ThyX chain A


gp48 D29p06 chain

 From SnapGene

"Thymidylate synthase complementing protein; Region: Thy1; pfam02511"





comparison between gp48 D29p06 and thymidylate synthase ThyX chain A



Alignment by Jaligner