information

Whoever comes in this website may find a hint

Phage therapy is influenced by:

Phage therapy is influenced by:

Country :
the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: a lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Chronolability
Mutation rate
Phenotypical delay
Phage cocktail
My point of view

From Wikipedia


If the target host* of a phage therapy treatment is not
an animal the term "
biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

"In silico"

From:"Genomics,Proteomics and Clinical Bacteriology", N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.

Saturday, 16 August 2014

D29p01


CDS:     401-1213, 813 bp, gp1: predicted 30.3 kD protein (270 Aa)

misc_feature: 461-1048,588 bp, Domain of unknown function
(DUF4417);Region: DUF4417; pfam14386

  By Snapgene software:



By Artemis software:

 
 Domain of unknown function (DUF4417) 
"This family of proteins is functionally uncharacterised. This family of proteins is found in bacteria and viruses. Proteins in this family are typically between 220 and 340 amino acids in length. There is a single completely conserved residue G (glycine) that may be functionally important".

 Pfam 
 Is a database of protein families (a protein family is a group of evolutionarily-related proteins, and is often nearly synonymous with gene family) that includes their annotations and multiple sequence alignments generated using hidden Markov models.


misc_feature Aa sequence:



Aa sequence (270 Aa):








-No helix-turn-helix nucleic acid binding motifs
 
-No prediction of transmembrane segments in protein 

- one PEST motif as potential proteolytic cleavage site 





- one site of cleavage between a signal sequence and the "mature exported protein"




  hypothetical "sequence" after cuttings



Virtual 2D-gel:



Is there a relationship with gp69 ?

CDS: 44,061-44,870, 810 bp, gp69: predicted 30.7 kD protein(269 Aa)

misc_feature: 44,262-44,843,582 bp, PD-(D/E)XK nuclease superfamily;Region:   PDDEXK_1; pfam12705

 statistics on the protein properties:


 
here

"PD-(D/E)XK nucleases comprise a large and extremely diverse group of proteins that are involved in various processes of nucleic acid metabolism. Typically, different PD-(D/E)XK families share little or no recognizable sequence similarity except for the conserved signature of the active site. As it happens, the name of this large group of nucleases derives from the highly conserved ‘PD’ (in many cases only ‘D’) and ‘(D/E)XK’ (‘X’ denotes the non-conserved position) active site motifs".

 here
  
My contribution:
in practice I verify if there is the Catalytic motif (P)D...Xn...(D/E)XK (where X is any aminoacid) in the sequences.

by Artemis software I download the  Fasta sequence of gp1 CDS and by Jemboss software  I analyse the Aa sequence:


!!!!!!!there are two catalytic motifs.


In the same way for gp69


!!!!!!!there are two catalytic motifs.

gp1 and gp69 have two Catalytic motif (P)D...Xn...(D/E)XK. One catalytic motif :   DX12(DXK)   in the sequences is in the same lenght.

Dot plot by Gepard software:








Deduction:
gp1 could be a protein with the same activity of  the protein gp69.