Mycobacterium phage L5 genome is used as example:
1-download the L5 mycobacterium phage genome (file.gbk from NCBI) or your genome (file.gbk).
2-open this file by Artemis software ( open source) or by another software.
3- each time select from Artemis software one gene and copy the corresponding protein sequence with fasta Aa.
4-paste and filter this sequence in Sequence Manipulation Suite ( open source) if is necessary , copy the filtrate protein sequence .
5- open mJemboss software (open source) and paste the sequence in Helix-Turn-Helix program.
7-safe the result.
8 - in Jemboss by the same sequence open other programs.
9- align the sequences by Strap software (open source) or by Jalview software (open source) and plot the Tree.
L5p36 (c26727-26557, 56Aa)
L5p44(c30476-28689, 595Aa)
L5p71(c44882-44331, 183Aa)
TREE
Alignment