information

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Phage therapy is influenced by:

Phage therapy is influenced by:

Country :
the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: a lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Chronolability
Mutation rate
Phenotypical delay
Phage cocktail
My point of view

From Wikipedia


If the target host* of a phage therapy treatment is not
an animal the term "
biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

"In silico"

From:"Genomics,Proteomics and Clinical Bacteriology", N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.

Saturday, 23 August 2014

How to identify Nucleic Acid binding Motifs in Protein Sequences


 Mycobacterium phage L5 genome is used as example:

1-download the L5 mycobacterium phage genome (file.gbk from NCBI) or your genome (file.gbk).

2-open this file by Artemis software ( open source) or by another software.

3- each time select from Artemis software one gene and copy the corresponding protein sequence  with fasta Aa.

4-paste and filter this sequence in Sequence Manipulation Suite ( open source) if is necessary , copy the filtrate protein sequence .

5- open mJemboss software (open source) and paste the sequence in Helix-Turn-Helix program.

7-safe the result.

8 - in Jemboss by the same sequence open other programs.

9- align the sequences by Strap software (open source) or by Jalview software (open source) and plot the Tree.
L5p46(c31389-30961,142 Aa)
In L5 mycobacterium phage proteome are present for example these Helix-turn-helix motifs :


L5p36 (c26727-26557, 56Aa)




L5p44(c30476-28689, 595Aa)


 











 L5p71(c44882-44331, 183Aa)

TREE












 


















Alignment