information

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Phage therapy is influenced by:

Phage therapy is influenced by:

Country :
the epidemiological situation is different from country to country in terms of circulating bacteria and bacteriophages. Example: a lytic phages from Italy may be no active on the same bacteria (genus and species) isolated from another country and vice versa.
Chronolability
Mutation rate
Phenotypical delay
Phage cocktail
My point of view

From Wikipedia


If the target host* of a phage therapy treatment is not
an animal the term "
biocontrol" (as in phage-mediated biocontrol of bacteria) is usually employed, rather than "phage therapy".

"In silico"

From:"Genomics,Proteomics and Clinical Bacteriology", N.Woodford and Alan P.Johnson

Phrase that emphasizes the fact that many molecular biologists spend increasing amounts of their time in front of a computer screen, generating hypotheses that can subsequently be tested and (hopefully) confirmed in the laboratory.

Saturday, 21 June 2014

Phage therapy conditions

Phage therapy may be used in those conditions  where the therapeutic success is elevated a priori.


Why?



a-Because Phage Therapy  maintains its best advantage, the RAPIDITY:
 "GO, KILL and  DISAPPEAR".


b- Because Phages must be Lytic and not modified by genetic engineering.


c-Because when we use the natural lytic phages in this SHORT TIME  IS IMPOSSIBLE to have:
 - a bacterial resistance that stops a phage attack ( 12 generations are necessary after a mutation of phage receptor in one bacterium for one phage and for this raison we use a minimum  of two phages in the cocktail).
- an active  immunological reaction ( two weeks are necessary).


d-Because it  is hard now to select phages and to prepare a phage cocktail by natural phages we can imagine how this difficulty will be high  with  engineered phages.

 e-Because with recombinant phages it is impossible to predict what will happen regarding the therapy and also  the phage preparation  is complicated and  the use of these phages do not avoid  the appearance of Phage Resistance.

 f-The effect of the Endotoxin    for example in Phage therapy in Cystic fibrosis disease could  be
prevented by  the use of Polimixina B.
If the points mentioned above are not adequately respected we must not use Phage Therapy .