D29p02

CDS :    1327-2106, 780 bp,  : predicted 28.8 kD protein (259 Aa)

misc_feature: 1570-1815, 246 bp, Protein of unknown function (DUF3626); Region: DUF3626; pfam12294    
 
By Snapgen software:
                                                     

By Artemis software:

Protein of unknown function (DUF3626)

"This family of proteins is found in bacteria. Proteins in this family are typically between 294 and 374 amino acids in length".

 misc_feature Aa sequence:

Aa sequence (259 Aa):

Statistics on the protein properties:

-No helix-turn-helix nucleic acid binding motifs
 
-No prediction of transmembrane segments in protein 

- No PEST motif as potential proteolytic cleavage site 

- N0 site of cleavage between a signal sequence and the "mature exported protein"

Virtual 2D-gel:

Is there a relationship with gp12? :

CDS:            6606-7370,765bp, gp12 :predicted 28.5 kD protein (254 Aa)
misc_feature: 6726-6983,258bp,Cutinase; Region: Cutinase; pfam01083


Statistics on the protein properties:

Cutinase and cutin is the substrate

1- CUTINASE_1, PS00155; Cutinase, serine active site  (PATTERN)

 Consensus pattern:
P-x-[STA]-x-[LIV]-[IVT]-x-[GS]-G-Y-S-[QL]-G
S is an active site residue

2- CUTINASE_2, PS00931; Cutinase, aspartate and histidine active sites  (PATTERN)

 Consensus pattern:
C-x(3)-D-x-[IV]-C-x-G-[GST]-x(2)-[LIVM]-x(2,3)-H
D and H are active site residues


My contribution:

by Artemis software I download the  Fasta sequence of CDS of gp12 and by Jemboss software  I analyse the Aa sequence in search for these consensus patterns:

 

There is  in gp12 only one consensus pattern: PYAX5GYSQG is inside  of the cutinase region and gp12 is the Lysin B of Mycobacteriophage D29.

 In the hypothetical protein from D29p02:
 

There is not consensus for cutinase activity but there is one motif for a nuclease activity:D X5 (DAK)

Deduction
The hypothetical protein from D29p02 is not correlated  to the gp12 protein.