Monday, 1 September 2014

D29p04



CDS :   2354-2650, 297 bp,  gp4 predicted 11.1 kD protein (98 Aa)
misc_feature: 2405-2569, 165 bp,HNH nucleases; HNH endonuclease signature which is found in viral, prokaryotic, and eukaryotic proteins. The alignment includes members of the large group of homing endonucleases, yeast intron 1 protein, MutS, as well as bacterial colicins, pyocins, and...; Region: HNHc; cd00085
misc_feature:2486-2569,active site


By Snapgen software:



By Artemis software:



Aa sequence (98Aa):


misc_feature  HNH nucleases, Aa sequence ( 55Aa):





 misc_feature ;active site,Aa sequence :DDHKPNLCRHS


misc-features: start and end:



 HNH nucleases




HNH motifs:







 consensus: e-x-h-H-i-x-p-x(2,4)-g-x(5,6)-N-l-x(3)-t-p-x(2,3)-H-x(3)-h ; capitalized letters are most conserved.


Scientific works: 





My contribution
By mJemboss software 

Statistics on the protein properties:

  
 one signal cleavage site in  the protein sequence:
 

Poor Pest motif:



Virtual 2G-gel:





In the sequence there are two motifs:

-DVDHK ( D/E X)K


-HSRSNLQAICRVCH


consensus:e-x-h-H-i-x-p-x(2,4)-g-x(5,6)-N-l-x(3)-t-p-x(2,3)-H-x(3)-h